L-Glutamate retrieved with the moulting fluid is processed by a glutamine synthetase in the pupal midgut of Calpodes ethlius.

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.

Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms.

1. Aspergillus nidulans, Neurospora crassa and Escherichia coli were grown on media containing a range of concentrations of nitrate, or ammonia, or urea, or l-glutamate, or l-glutamine as the sole source of nitrogen and the glutamate dehydrogenate and glutamine synthetase of the cells measured. 2. Aspergillus, Neurospora and Escherichia coli cells, grown on l-glutamate or on high concentrations...

Differential ammonia metabolism in Aedes aegypti fat body and midgut tissues.

In order to understand at the tissue level how Aedes aegypti copes with toxic ammonia concentrations that result from the rapid metabolism of blood meal proteins, we investigated the incorporation of (15)N from (15)NH(4)Cl into amino acids using an in vitro tissue culture system. Fat body or midgut tissues from female mosquitoes were incubated in an Aedes saline solution supplemented with gluco...

Genesis of Mitochondria in Insect Fat Body

Electron microscopy and stereological methods have been used to study the time course and mechanism of mitochondrial genesis in the adult fat body of Calpodes ethlius, (Lepidoptera, Hesperiidae). Most of the larval mitochondria are destroyed during a phase of autolysis shortly before pupation, so that pupal and early adult fat body cells have few mitochondria. The number of mitochondria per cel...

The regulation of glutamine synthesis in the food yeast Candida utilis: the purification and subunit structure of glutamine synthetase and aspects of enzyme deactivation.

Yeast glutamine synthetase was purified and shown to be an octameric globular protein (s = 15.4,fF0 = 1-29, mol. wt = 390000). It consists of two weakly-bound half molecules (s = 8.7, fFo = 1-35, mol. wt = 180500) and relatively harsh treatment is required to dissociate these octamers into component monomers (s = 3.8). Deactivation of the enzyme in vivo may include changes in the conformation o...